Curricular Unit:			Code:
General Microbiology			853MGER
Year:	Level:	Course:	Credits:
1	Undergraduate	Nursing	5 ects
Learning Period:		Language of Instruction:	Total Hours:
		Portuguese/English	65
Learning Outcomes of the Curricular Unit:
Objectives: Identify the structural features of prokaryotic, eukaryotic cells and viruses; Identify the factors that influence the growth and metabolism of microorganisms. Describe the general principles of microbial genetics. Identify and describe the mechanisms of action of antibiotics and antiviral. Identify and describe the basic mechanisms of immune response and its modulation strategies. Properly perform aseptic technique; Using the optical microscope correctly; Understand the different staining techniques for the visualization of microorganisms; Understand and distinguish the different methods for microbial identification; Perform and correctly interpret an antibiogram. Required skills : Acquire habits of observation and critical analysis within Microbiology; Take decisions based on available scientific knowledge; Analyze the bibliographic information critically.
Syllabus:
1. History of Microbiology 1.1. Major events and personalities in the field of Microbiology 2. Diverse roles of microorganisms 3. The main groups of microorganisms: Bacteria, Fungi, Protozoa and Viruses. 3.1 Cellular organization of microorganisms. 3.1.1. Prokaryotes 3.1.1.1. Bacteria 3.1.1.2. Archaea 3.1.2. Eukaryotes 3.2. Virus 3.2.1 Ultra structure 3.2.2 Viral Cycle 4. Microbial Genetics 4.1. Nucleic acids 4.2. Diversity of the microbial world 5. Nutrition and growth of microorganisms 5.1. Nutritional requirements 5.1.1. Culture media 5.2. Multiplication and death of microbial populations 5.2.1. Growth kinetics. 5.2.2. Factors affecting bacterial growth 6. Antimicrobial agents 6.1. Biocides 6. Antimicrobial agents 6.2. Antibiotics 7. Host/Parasite Relationship 7.1. Commensal flora 7.2. Mechanisms of host defense 7.2.1. Primary defenses 7.2.2. Immune system 7.2.2.1. Innate system 7.2.2.2. Adaptive system 7.3. Microbial virulence factors
Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:
The syllabus presented allow the knowledge of the various aspects of microbiology. In addition to an introduction of environmental and historical context, it is made a presentation of the basic structures of microorganisms to allow in the future to associate their characteristics with possible effects on humans and the environment that surrounds us. The study of the genetic component is associated with the development of processes which allow an adaptation from microorganisms with greater or lesser effectiveness, to the control mechanisms, developed and used by man. Finally students are exposed to a vast and varied number of interactions between microorganisms and their human hosts, ranging from the ability of microorganisms to survive and the defence capabilities of its hosts. These syllabuses have a high relevance to the proposed objectives, enabling these resources to relate the various constituents of microorganisms with their effects on humans.
Teaching Methodologies (Including Evaluation):
Interrogative, Demonstrative and Expository Oral presentation of the syllabus. Active / participatory methodology, with support of media. Bibliographic research with support by the faculty. Laboratory simulation procedures, with analysis, interpretation and discussion of results. Assessment: Assessment follows the determined in the "Normativa Académica de Funcionamento das Licenciaturas e Mestrados Integrados da UFP" Theoretical Component (T): Two assessment tests that focus on the course syllabus. Practical component (P): Two tests of individual practical assessment. Final Rating: FR = [((T1+T2)/2)x 0,8] + [((P1+P2)/2) x 0,2] The student must obtain all ECTS in order to be approved. It is a regulatory condition to approval that the student's attendance is at least 50% of lectures and 80% of the practical classes.
Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:
During the lectures, the need to analyze and reflect on new concepts involves the use of expository and interrogatory methods, leaving space for the active participation of students in order to settle the new knowledge linking it to already established ideas. The simulation of practical procedures in microbiology allows for the consolidation of theoretical knowledge through scientific experimentation so enhancing new levels of understanding. The practical component also has the virtue of exposing students to reality of the existence of microorganisms and their ability to survive in our ecosystem. This exposure aims to make students understand the need for interaction and control of microorganisms in the development of their professional life. The realization of two moments of theoretical evaluation to validate the acquisition of knowledge as well as assessment of the evolution of the students in the ability to apply the acquired knowledge. The evaluation conducted in laboratory setting, through practical tests, confirms the technical competence in performing laboratory procedures and identify the level of acknowledgment of the risks associated with different procedures.
Reading:
(1) Ferreira, W.F.C. and Sousa, J.C. – Microbiologia – LIDEL, 2010 (2) Willey, J.M., Sherwood, L.M., Woolverton, C.J. – Prescott's Microbiology – McGraw-Hill, 10ª edição, 2016 (3) Sousa, J.C. – Antibióticos volume 1 – Edições UFP, 2016. (4) Sousa, J.C., Cerqueira, F., Abreu, C. – Microbiologia. Protocolos laboratoriais, 2ª edição– Edições UFP, 2012.