Curricular Unit:Code:
Spectophotometric Techniques1100TESP
1CTSPLaboratory Analyses6 ects
Learning Period:Language of Instruction:Total Hours:
Spring SemesterPortuguese/English78
Learning Outcomes of the Curricular Unit:
Spectrophotometry is a widely used analytical technique that stands out for its simplicity, versatility, reliability and speed. This course has as its main objective the acquisition of the fundamental principles of spectrophotometric techniques and the adaptation of this knowledge to laboratory practice. The skills to be developed are based on knowledge of the aspects inherent to quantitative spectrophotometric analysis, which will serve to understand and perform the activities performed in the laboratory. The objectives to be achieved are:
O1. Identify the scope and application of spectrophotometry
O2. Know the fundamentals of spectroscopy
O3. Acquire the theoretical basis inherent to the treatment and statistical evaluation of data
O4. Understand the assumptions for the application of Beer's Law
O5. Understand the components and operation fundamentals of a UV-Vis spectrophotometer
O6. Develop laboratorial skills to perform an analysis by atomic spectroscopy
1. Introduction to spectrophotometric methods
2. Fundamentals of spectrophotometry
2.1 Properties of electromagnetic radiation
2.2. The electromagnetic spectrum
2.3 Interaction of electromagnetic radiation with matter
2.3. absorption of electromagnetic radiation
3. Treatment and statistical evaluation of data
3.1. General analytical performance characteristics
3.2 Calibrations and quantitative calculations - standardization methods
4. Beer's law in chemical analysis
4.1. Applicability conditions of Beer's law
4.2. Lambert-Beer's law
4.3 Real limitations, chemical limitations and instrumental deviations
5. Ultraviolet-visible spectrophotometry
5.1 Instruments for molecular optical spectroscopy
5.2. Types of UV/Vis spectrophotometers - mode of operation
6. Atomic spectroscopy
6.1 Equipment - general aspects
6.2. Flame atomic emission spectroscopy (FEA)
6.3. Emission plasma spectroscopy (ICP)
Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:
The syllabus (CP) of the Analytical Chemistry Curricular Unit provides students with the acquisition of scientific, technical and interpersonal skills that allow them to develop their future professional laboratory activity in this area. PC1, PC2 and PC4 aim to achieve objectives O1 and O2. CP3 and CP4 serve to achieve objectives O3 and O4. The CP’s involving the different types of instruments (CP5-6) are designed to achieve goals O3, O5, and O6.
Teaching Methodologies (Including Evaluation):
M1: Exposition and debate of relevant theoretical concepts in the classroom. Didactic material will be made available on the Canvas platform.
M2: Orientation of students' autonomous study in the practical component. In theoretical and practical classrooms, students will be asked to solve a series of questions with an objective answer and numerical problems.
M3: Active participation of the student in the teaching-learning process through the execution of a set of laboratory works.
Assessment: the acquisition of knowledge of the theoretical-practical component is validated by carrying out 2 written tests (with a weighting coefficient of 40% in the final grade of the course). The evaluation of the practical component (with a weighting coefficient of 60% in the final grade) will be based on laboratory performance and on the quality of the worksheets delivered (50%) and on 2 written tests (50%). The final classification will only be awarded when both components are approved.
Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:
The constant interaction between the teacher and the student in class will allow the student to adapt to the proposed objectives. In the theoretical-practical classes, the general principles inherent to spectrophotometric analysis are exposed, ranging from the preparation and manipulation of solutions to the fundamental operations for the execution of an analytical procedure. The different types of quantification methods, their characteristics, advantages and disadvantages are studied. In the laboratory classes the student will consolidate the topics covered in the theoretical-practical component and become familiar with the practice of spectrophotometric analysis by performing a series of laboratory tasks. The methodologies (M) defined are articulated with the objectives (O) proposed: M1: Exposition and debate of relevant theoretical concepts in the classroom - Aims to achieve O1-O6. M2: Orientation of students' autonomous study in the practical component - Aims to achieve O3-6. M3: Active participation of the student in the teaching-learning process through the execution of a set of laboratory works - Aims to achieve O3, O5 and O6.
Harris, D. C. e Lucy C. A. Quantitative Chemical Analysis, 10ª ed., W. H. Freeman, 2019. ISBN: 978-1319164300.
Christian, G. D., Dasgupta, P. K. e Schug, A. Analytical Chemistry, 7ª ed, Wiley, 2013. ISBN: 978-0470887578.
Skoog D. A., West D. M., Holler, F. J. e Crouch S. R. Fundamentals of Analytical Chemistry, 9ª ed, Cengage Learning, 2021. ISBN: 978-0357450390.
Skoog, D. A., West, D. M., Holler, F. J. e Crouch, S. R. Fundamentos de Química Analítica, Tradução da 9ª ed. norte-americana, Cengage Learning, 2014. ISBN: 978-8522116607.
Lecturer (* Responsible):
Renata Souto (