Curricular Unit:			Code:
Chromatographic Techniques			1100TCRO
Year:	Level:	Course:	Credits:
2	CTSP	Laboratory Analyses	6 ects
Learning Period:		Language of Instruction:	Total Hours:
Winter Semester		Portuguese/English	78
Learning Outcomes of the Curricular Unit:
OA1: Promotion of knowledge of basic concepts of chromatography and their use in the understanding of various chromatographic techniques. OA2: Study of the various types of chromatographic techniques, including chromatographic systems with their instrumental components and practical applications. OA3: Development of an analytical and critical mentality in relation to the different techniques in order to value their correct use and understanding, in other learning blocks and in future professional practice.
Syllabus:
CP1: Chromatographic separations introductory concepts. Definition of chromatography. Types of Chromatography - Paper, Plate and Column. Mobile phase, stationary phase, eluent, elution. Main mechanisms of chromatographic separation processes: partitioning, adsorption, ion exchange, molecular exclusion, affinity. CP2: The chromatogram. Retention time, capacity and selectivity factors, chromatographic resolution and efficiency. CP3: Chromatographic Systems. Gas chromatography: injection systems, columns, detectors, and quantitative analysis. High performance liquid chromatography (HPLC): columns, solvents used, detectors, gradient elution operation and quantitative analysis.
Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:
The syllabus of the course was divided into several chapters covering different aspects of interest in chromatographic techniques for laboratory analysis. In addition, the student will also have the opportunity to put theoretical knowledge into practice through the execution of laboratory work on chromatographic techniques. The syllabus (CP) were delineated according to the learning objectives (LO) of the curricular unit. To reach OA1 and OA3 the student will have to acquire a satisfactory level of knowledge in all syllabus contents. The matters covered in CP2 aim to respond to OA1. OA2 will be achieved if the student acquires a satisfactory level of knowledge of the CP3 of the syllabus.
Teaching Methodologies (Including Evaluation):
M1: Presentation and oral explanation of the syllabus, with the support of audiovisual media. M2: Call for active participation by students, through an interrogative methodology. M3: Solving exercises. M4: Execution of laboratory work. The minimum percentage of attendance of the theoretical-practical classes of this curricular unit is 50%, in accordance with the Pedagogical Regulation in force at UFP. The assessment is periodic with two tests in the theoretical-practical component and two written tests in the practical-laboratory component, both with a weighting of 50%. Approval for the curricular unit implies global approval for both components, whose weight is 80% for TP and 20% for PL.
Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:
The application of the teaching methodologies adopted for the theoretical-practical component aims at acquiring and consolidating knowledge in the technical-scientific domain of the course, improving the ability to apply concepts to the resolution of practical problems in Laboratory Analysis and guiding the student towards autonomous learning. Thus, at an early stage, the oral exposition and explanation (M1) enable the approach and understanding of the syllabus. Classes focus on active and participatory methods, with a view to keeping students' attention and developing their critical capacity (M2). The solving of exercises leads to greater motivation of students in the application and consolidation of acquired knowledge (M3). The appeal to students' critical sense in the analysis of results enhances their ability to interconnect knowledge and properly interpret the results obtained. The execution of laboratory work allows the student to learn by doing (M4).
Reading:
D. Skoog, F. Holler, S. Crouch. Principles of Instrumental Analysis. 7th edition, Cengage Learning (2017) M.L. Sadler Simões Gonçalves. Métodos Instrumentais para Análise de Soluções. 4ª ed., Fundação Calouste Gulbenkian (2001) H.H. Willard, L.L. Merritt Jr. And J.A. Dean. Instrumental Methods of Analysis. 7 th Ed. New York, Wadsworth (1988) D. Skoog, D. West, F. Holler, S. Crouch. Fundamental of Analytical Chemistry. 9th Edition. Cengage Learning (2013)
Lecturer (* Responsible):
Sérgio Barreira (barreira@ufp.edu.pt)