|Molecular and Cellular Biology||842BMC|
|Learning Period:||Language of Instruction:||Total Hours:|
|Learning Outcomes of the Curricular Unit:|
|Molecular and cellular biology approaches living organisms from a unifying perspective, the cell, integrating knowledge from various disciplinary areas. Thus, the learning process aims at the acquisition of (1) knowledge to allow a deep understanding of the molecular basis of cell physiology; (2) general scientific skills – (i) analysis, interpretation and validation of technical and scientific data, (ii) communication of scientific information, and (iii) autonomy to update knowledge. At the competency level, the learning process provides the ability to recognize and understand the cell’s role in health and disease.|
|I. Theoretical: Historical perspective; Structural matrix (cell and water); Biomembranes (Ultra-structure, composition-structure and function of lipids and proteins (glycoproteins and enzymes), general characteristics and functions-transport and biosignalling); Cytoskeleton and extracellular matrix-structural relationship with biomembranes; Cell organelles (ultrastructure, chromatin and DNA, RNA synthesis and transport, biogenesis-soluble proteins, and pathophysiology); Proteostasis (Mechanisms, network and response), Cell proliferation (cell cycle and its regulation); Cell death. (Classification, characteristics and mechanisms).|
II. Laboratory practice: Safety, equipment and good laboratory practices; Optical microscopy and micrometry: plant tissues, animal tissues and prokaryotic cells; Structural analysis of amino acids and proteins; Protein isolation; Study of catalase action; Chloroplast isolation; Studies on the permeability of biomembranes.
|Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:|
|The theoretical component analyses the cell from a molecular perspective with description of diseases caused by loss or gain function of proteins, promoting a broad and integrated understanding of cellular physiopathology. The practical component is focused on problem solving, allowing the student to contact with basic experimental techniques, apply the scientific method, apply theoretical concepts, and develop the method and practice of bibliographic research. The interconnection and complementarity between these components promotes and improves the: (i) Knowledge of the cell role in the dynamics of multicellular organisms, in health and disease; (ii) Ability to implement basic techniques and interpret experimental data; (iii) Analysis and comprehension of scientific literature; (iv) Development of scientific reasoning; (v) Apprehension and communication of technical-scientific information. Thus, the execution of the syllabus will generate the intended learning outcomes.|
|Teaching Methodologies (Including Evaluation):|
|Theoretical component - 80% of the final grade obtained through the weighted average of two written evaluations (minimum grade of 10/20 values), individual and without consultation. Laboratory practice component: 20% of the final grade obtained as follows: 18% of the final grade (minimum grade of 10/20 values) of the evaluations (two theoretical-practical), individually carried out and without consultation, each with a weighting of 6% and 12%; the additional 2% is obtained through the presentation of the laboratory activity. Teaching material and scientific articles or other free use materials will be available through e-learning. The curricular unit will have a single final classification, so it will only be awarded when both components are approved. Failure in the theoretical component sends the student to exam.|
|Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:|
|I. Theoretical component: The component is developed from a descriptive and integrated exposition of the concepts, theories and mechanisms underlying the theoretical program. Its progression occurs in articulated way with the didactic material and the bibliography, and includes the joint reflection on key aspects of the programme content. For the acquisition of knowledge, direct and indirect teaching methodologies are applied, which simultaneously promote a broad and integrated level of understanding and improvement of self-learning. II. Practical component: Learning is based on practical problem solving, using an experimental or practical approach, and aims at improving: (i) Conceptual integration, (ii) Operationalization in a laboratory environment (organization, reasoning and execution), (iii) Collection, selection, analysis, systematization, critical interpretation and validation of technical-scientific information, (iv) Oral and written communication of scientific information, and (v) Ability to independently update knowledge. Self-learning will also be stimulated through the development of a bibliographic research work by groups of 2-3 students aiming to support the critical review presentation of an experimental work. Courseware, including computer-aided teaching material and supporting texts (e.g. Exercise Manual with scientific background, learning objectives for each module of the theoretical program and solved exercises, and Practical-Laboratory Handbook, both developed by teachers), and scientific articles or articles of free use will be available through e-learning. In addition to the contact hours of the academic periods of the course, the learning process can be complemented during the office hours of the teacher, or using the digital institutional communication tools (e-mail and e-learning). The simultaneous use of these institutional computing resources for the dissemination of either didactic material or scientific literature also represents an important contribution to the promotion and stimulation of the student self-learning process.|
|1.QUINTAS A., FREIRE A.P., HALPERN M.J. – Bioquímica: Organização molecular da vida/Lidel, 2008|
2.MURRAY RK, GRANNER DK, RODWELL VW – Harpers’s Illustrated Biochemistry – Lange Medical Books/McGraw-Hill, 27th ed, 2006
3. SUNKEL CE, AZEVEDO C. - Biologia Molecular e Celular, Lidel eds. Técnicas, 5ª Edição, 2012 ISBN: 9789727576920 (existe 4ª edição, 2010)
4. RIBEIRO MG et al. - Manual de exercícios de apoio à componente teórica da UC, 2014.
5. CASTRO A, CASTRO R, COELHO MJ, RIBEIRO MG Manual de trabalhos laboratoriais de apoio à componente prática da UC, 2017.
6. Lodish H, Berk A, Kaiser CA, Krieger M, Bretscher A, Ploegh H, Amon A, Martin KC. Molecular Cell Biology. WH Freeman, 8th Edition, 2016. ISBN: 9781464183393. (existe a 3ª edição, 1995, e a 4ª edição, 2000)
7. Alberts B, Johnson A, Lewis J, Morgan D, Raff M, Roberts K, Walter P. Molecular Biology of the Cell. Garland Science, 5th Edition, 2014. ISBN: 9780815344322. (existe a 4ª edição, 2002)