Curricular Unit:Code:
Instrumental Methods of Analysis843MIA
2MasterPharmaceutical Sciences4 ects
Learning Period:Language of Instruction:Total Hours:
Winter SemesterPortuguese/English52
Learning Outcomes of the Curricular Unit:
Most routine analytical practices used in health sciences, food and environmental sciences and numerous industrial areas are now based on instrumental methods of analysis. In fact, the determination of analytical species through classical methods has become less relevant because they lack sensitivity, selectivity and speed and their precision is easily altered. The aim of this course is to describe some of the analytical instruments most frequently used. Specificities of each equipment, their application and the precautions that need to be considered for its correct use are carefully examined.
1. Introduction to Instrumental methods of analysis
2. Quantitative aspects of spectrochemical analysis
3. UV/Vis spectrophotometry
4. Atomic spectroscopy
5. Potentiometry
6. General aspects of chromatographic separations
7. Liquid Chromatography
8. Gas Chromatography
Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:
Analytical procedures, now used in health sciences and in numerous industrial areas, are based on the so-called instrumental methods of analysis. Chemical analysis is now based on spectrophotometric, potentiometric or chromatographic methodologies.
This course seeks to provide a solid theoretical background on the main principles regarding different analytical techniques. This training is then enhanced in practical classes where the student has to perform several relevant determinations, having the opportunity to use all the instruments studied. This course enhances students' laboratory performance enabling them to develop skills that allow the acquisition of good quality results and also enables them to judge the quality of other experimental results that they may have to consider.
Teaching Methodologies (Including Evaluation):
Methodologies (M): (M1) In the theoretical classes, the relevant theoretical concepts are exposed and discussed and guidance for autonomous study is given. Common laboratory instruments are studied carefully resorting to the use of graphs and images. (M2) These contents are consolidated in practical classes dedicated to the resolution of questions of objective answer and numerical problems that allow the clarification of doubts and the deepening of specific topics. (M3) Active participation of the student in the teaching-learning process through the execution of a set of laboratory works.
Assessment: the acquisition of knowledge of the theoretical component is validated through two written tests (70% of the final grade). The evaluation of the practical component (30% in the final grade) will be based on laboratory performance, the quality of the results sheets delivered (50%) and two written tests (50%). The final classification will only be awarded if both components are approved.
Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:
At the end of this course, students should be able to recognize and describe some of the most important analytical tools used in contemporary quantitative analysis. Main operation principles of several methodologies should be fully understood and the main components of each instrument and its characteristics easily described. The knowledge of each equipment allows information of its limitations/specificities and enables the careful and strict control of all instrumental parameters that limit the quality of analysis. Experimental treatment of data obtained should be clearly understood in order to appropriately process analytical signals and obtain results with adequate precision and accuracy. Thus being, the course of Instrumental Methods of Analysis is divided into two essential components. In the theoretical part the concepts inherent to physical and chemical phenomena involved in instrumental analysis (ranging from electronics, electrochemical to optical light processes) are taught. In the laboratory practice component, students will perform several quantitative determinations using spectrophotometric, potentiometric and chromatographic procedures, thus having the opportunity to work and learn about all these instruments.
Christian, G. D., Dasgupta, P. K. e Schug, A., Analytical Chemistry, 7ª ed, Wiley, 2013. ISBN: 978-0470887578.
Skoog, D. A., Holler, F. J. e Crouch, S. R., Principles of Instrumental Analysis, 7ª ed, Cengage Learning, 2017. ISBN: 978-1305577213.
Harris, D. C. e Lucy C. A. Quantitative Chemical Analysis, 10ª ed., W. H. Freeman, 2019. ISBN: 978-1319164300.
Skoog D. A., West D. M., Holler, F. J. e Crouch S. R. Fundamentals of Analytical Chemistry, 9ª ed, Cengage Learning, 2013. ISBN: 978-0495558286.
Lecturer (* Responsible):
Renata Souto (