Curricular Unit:Code:
General Microbiology852MGER
Year:Level:Course:Credits:
1UndergraduateNursing5 ects
Learning Period:Language of Instruction:Total Hours:
Portuguese/English65
Learning Outcomes of the Curricular Unit:
This course unit of General Microbiology's main purpose is to provide undergraduate nursing students to acquire scientific and technical skills that enable them to learn the fundamentals of microbiology, including the structure and biology of microorganisms, microbial diversity and ecology, control of microbial growth, the relationship of microorganisms to man, as well as basic laboratory techniques in microbiology.
Thus, the student must meet the following objectives:
Distinguish a prokaryotic cell from an eukaryotic cell;
Identify the structural features of prokaryotic, eukaryotic cells and viruses;
Identify the factors that influence the growth and metabolism of microorganisms.
Describe the general principles of microbial genetics.
Identify and describe the mechanisms of action of antibiotics and antiviral.
Identify and describe the basic mechanisms of immune response and its modulation strategies (eg vaccination)
Properly perform aseptic technique; Using the optical microscope corr
Syllabus:
1. History of Microbiology
1.1. Major events and personalities in the field of Microbiology
2. Diverse roles of microorganisms
3. The main groups of microorganisms: Bacteria, Fungi, Protozoa and Viruses.
3.1 Cellular organization of microorganisms.
3.1.1. Prokaryotes
3.1.1.1. Bacteria
3.1.1.2. Archaea
3.1.2. Eukaryotes
3.2. Virus
3.2.1 Ultra structure
3.2.2 Viral Cycle
4. Microbial Genetics
4.1. Nucleic acids
4.2. Diversity of the microbial world
5. Nutrition and growth of microorganisms
5.1. Nutritional requirements
5.1.1. Culture media
5.2. Multiplication and death of microbial populations
5.2.1. Growth kinetics.
5.2.2. Factors affecting bacterial growth
6. Antimicrobial agents
6.1. Biocides
6. Antimicrobial agents
6.2. Antibiotics
7. Host/Parasite Relationship
7.1. Commensal flora
7.2. Mechanisms of host defense
7.2.1. Primary defenses
7.2.2. Immune system
7.2.2.1. Innate system
7.2.2.2. Adaptive system
7.3. Microbial virulence factors
Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:
During the lectures, the need to analyze and reflect on new concepts involves the use of expository and interrogatory methods, leaving space for the active participation of students in order to settle the new knowledge linking it to already established ideas.
The simulation of practical procedures in microbiology allows for the consolidation of theoretical knowledge through scientific experimentation so enhancing new levels of understanding.
The practical component also has the virtue of exposing students to reality of the existence of microorganisms and their ability to survive in our ecosystem. This exposure aims to make students understand the need for interaction and control of microorganisms in the development of their professional life.
Teaching Methodologies (Including Evaluation):
Interrogative, Demonstrative and Expository
Oral presentation of the syllabus.
Active / participatory methodology, with support of media.
Bibliographic research with support by the faculty.
Simulation conditions in order to facilitate the realization of different techniques and laboratory procedures.
Experimental work in practical classes that implement the theoretical concepts, with analysis, interpretation and discussion of results, will be made.
The classroom component will consist of lectures and laboratory practices.
The evaluation of the course includes the following events:
Theoretical Component (TC): Two assessment tests that focus on the course syllabus.
Practical component (PC): Two tests of individual practical assessment (50% each assessment).
Individual Work (IW): A bibliographic research supported with a publishing component in electronic form.
Final Rating: FR = (TC x 0.6) + (PC x 0.2) + (IW x 0.2)
The student must obtain a minimum grade of 10 values on each component (T
Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:
The realization of two moments of theoretical evaluation to validate the acquisition of knowledge as well as assessment of the evolution of the students in the ability to apply the acquired knowledge. The evaluation conducted in laboratory setting, through practical tests, confirms the technical competence in performing laboratory procedures and identify the level of acknowledgment of the risks associated with different procedures. The evaluation of the Individual Work allows the validation of the student's ability to independently acquire knowledge, validate it, and finally, to communicate it effectively.
Reading:
(1) Ferreira, W.F.C. and Sousa, J.C., Lima, N. – Microbiologia – LIDEL, 2010
(2) Sousa, J.C. – Manual de antibióticos antibacterianos – 2ª edição – Edições UFP, 2007
(3) Sousa, J.C., Cerqueira, F., Abreu, C. – Microbiologia. Protocolos laboratoriais – Edições UFP, 2006
(4) Strohl, W.A., Rouse, H., Fisher, B. D. - Microbiologia Ilustrada – Artmed, 2004
(5) Cowan, M.K., - Microbiology: A Systems Approach - McGraw-Hill Science, 3 edition, 2011