Curricular Unit:Code:
Instrumentation and Automation1055IAUT
Year:Level:Course:Credits:
1MasterSpecialized Laboratory Analyses (Alimentary and Environmental Analysis)6 ects
Learning Period:Language of Instruction:Total Hours:
Winter SemesterPortuguese/English78
Learning Outcomes of the Curricular Unit:
Acquisition of knowledge about the instrumentation that is used today to carry out all types of analysis. Train the students in the use of this same instrumentation. The main skills that the student should achieve in this course are:
- understanding of the techniques and the instrumentation used to determine the analytes with relevance in the areas of clinical analysis, food analysis, environmental analysis and forensic analysis;
- Learning how to work with this same instrumentation;
- Learning how to plan and perform an analysis using instrumental techniques.
Syllabus:
1. Instrumentation for spectrophotometry
2. Instrumentation for chromatography
3. Instrumentation for electroanalytical techniques
4. Introduction to automated analyzers
5. Automated Analyzers for Clinical Chemistry
6. Instrumentation for the measurement of blood gases
7. Instrumentation for electrophoresis
8. Automated Hematology Analyzers
9. Automated Analyzers for Clinical Microbiology
10. Analyzers for immunochemistry
11. Applications in forensic analysis
12. Applications in environmental analysis
13. Automated instrumentation in the food industry
Demonstration of the Syllabus Coherence with the Curricular Unit's Objectives:
Today, the separation, identification and quantification of a given analyte is done essentially by means of chromatographic methods, the measurement of the interaction between matter and radiation (spectrophotometric techniques), the measurement of electrical properties (electroanalytical methods) or the measurement of mass / charge ratio (mass spectrometry). The first three chapters of the program deal with the presentation of the fundamentals of the above techniques and the instrumentation (components and equipment) that is necessary to implement them. Using only manual methods it is humanly impossible these days to respond timely to the needs of analysis in some areas, namely the clinic hence the need for automation. In ch. 4 the fundamentals of automated instrumentation are explained. In the remaining chapters (5 to 12) the specific instrumentation that is currently used for clinical, food, environmental and forensic analysis is presented.
Teaching Methodologies (Including Evaluation):
The classes of this course unit are divided into "theoretical classes" which are primarily intended for the presentation of the syllabus contents and in "laboratory classes" in which the student contacts the instrumentation directly during the execution of a set of analyzes.
These contents are consolidated by using a series of questions and numerical problems that are proposed to the students. The laboratory classes include the execution of a set of reports concerning the laboratory activities.
Demonstration of the Coherence between the Teaching Methodologies and the Learning Outcomes:
At the end of this course, students should be able to recognize and describe the most important instrumentation used in contemporary quantitative analysis. The treatment of the experimental data obtained must be fully understood so that an adequate processing of the data is possible, in order to obtain results with adequate precision and accuracy. In this sense, the curricular unit of Instrumentation and Automation is divided into two essential components. In the theoretical part are given concepts inherent to the physical and chemical phenomena involved in an instrumental analysis, instrumentation that takes advantage of these phenomena to obtain chemical information and automated instrumentation. In the practical laboratory component, the student will be called to carry out quantitative analyzes using spectrophotometric, potentiometric and chromatographic methods, having the opportunity to learn to work with the equipment used for this purpose, namely automated instrumentation.
Reading:
[1] - Kory M. Ward-Cook, Craig A. Lehmann, Larry E. Schoeff, Robert H. Williams; Clinical diagnostic technology, AACC Press; 2005
[2] - Kory M., Ph.D. Ward, Craig A. Lehmann, and Alan Leiken; Clinical laboratory instrumentation and automation. Saunders , 1994
[3] - Skoog, D.and Leary, J.; Principles of instrumental analysis, Saunders college publishing, 1992.
[4] - Gerald J. Kost (Ed). ;Handbook of Clinical Automation, Robotics, and Optimization. , Wiley-Interscience, 1996
[5] - Michael G. Bissell and John R. Petersen; Automated Integration of Clinical Laboratories: A Reference, AACC, 1998
[6] - Wayne R. P.; Chemical instrumentation. Oxford University Press, 1994